Plant biotechnology is prominent in the field of medicine interfacing biotechnology and bioinformatics, the molecular characterization of medicinal plants; molecular farming; and result from chemistry, nanotechnology, pharmacology, agriculture, Biomass and biofuels as well. Plant tissue culture is the growth of plant cells outermost an intact plant. It depends on maintaining plant tissue in lab conditions on a suitable nutrient medium. The culture can be sustained as a mass of undifferentiated cells for a broad area for a period of time or regenerated into whole plants. The dissimilar techniques used in plant tissue culture. Plant tissue culture is mostly used to produce clones of a plant in a method known as micro-propagation with different stages. Plant Biotechnology is the technology which is used for getting modern product with high yield and at faster rate.